BPC-157 Reconstitution Calculator

This calculator estimates the concentration of a BPC-157 preparation after combining a lyophilized powder with bacteriostatic water. Simply choose the vial size and the amount of water you aim to include, and the calculator will display the quantity of peptide contained in one milliliter of mixed solution. Although the math is easy, we have added it here to cut down on the chances that mistakes will be made while mixing the solution. This does not constitute dosage advice.

How the Calculator Works

The same mathematical equation is used by the calculator. It first converts milligrams of peptide into micrograms and then divides that number by the total water volume, and then -- finally, it multiplies the result by the draw volume. Hence, the output is the amount of peptide in a single draw. The equation presumes that the peptide powder dissolves completely and retains its full amount during the entire reconstitution period.

Concentration (mcg per 0.1 ml) = (peptide in mg x 1000) / (water in ml) x 0.1

We would like to clarify that the information presented here is related to unit conversion, not advice in any form. The output indicates the quantity of peptide in a specified volume of liquid. Any implications stemming from this knowledge belong to the user and are not the responsibility of this calculator.

Inputs: Vial Size and Water Volume

The calculator takes two inputs. The first is the vial size in milligrams, which corresponds to the amount of lyophilized peptide in the sealed vial. PeptideGod sells BPC-157 in 5 mg and 10 mg sizes. Real Peptides offers a 10 mg vial. The second input is the volume of bacteriostatic water you add during reconstitution, measured in milliliters. Common volumes range from 1 ml to 5 ml depending on the desired concentration.

Output: Concentration per Unit

The calculator returns the concentration in micrograms per 0.1 ml, which corresponds to one tick mark on a standard 1 ml insulin syringe. It also displays the concentration per full milliliter for reference. A higher water volume produces a more dilute solution. A lower water volume produces a more concentrated one. The total peptide content in the vial stays the same regardless of how much water is added.

Example Calculations

The following table illustrates the concentration outcomes based on the most popular formulations involving vials and water. Please note that all results assume non-loss of peptde during the dissolution process and hence obtaining full dissolution.

5 mg Vial with 2 ml Water

Start with 5 mg, which equals 5,000 micrograms. Divide by 2 ml to get 2,500 mcg per ml. Each 0.1 ml draw on a standard insulin syringe contains 250 mcg of BPC-157. This is one of the more common reconstitution setups because it balances concentration with a reasonable number of draws per vial. At 0.1 ml per draw, a 2 ml total volume provides 20 draws from the vial.

10 mg Vial with 2 ml Water

Begin with 10 mg which is equal to 10,000 micrograms. When divided by 2 ml, this gives 5,000 mcg per ml. Each of the 0.1 ml draws delivers 500 mcg of BPC-157. The increased peptide dose with each draw means that fewer total draws will be necessary for equal amounts compared to the 5 mg approach. While the total number of draws for the 0.1 ml amount remains 20, each draw will now provide twice the amount of peptoid.

What You Need

Bacteriostatic water is available from the same vendors that sell BPC-157. Real Peptides offers a 10 ml vial for $4.80 during their current sale. A single 10 ml vial provides enough water for multiple reconstitutions. At 2 ml per peptide vial, that covers five vials.

Step-by-Step Reconstitution Process

The procedure below employs normal aseptic technique as previously mentioned in peptide studies. It is applicable to any kind of lyophilized BPC-157 manufactured by any company.

1. Gather supplies Collect your lyophilized BPC-157 vial, bacteriostatic water, alcohol swabs, and an insulin syringe. Work on a clean, flat surface.
2. Wipe both stoppers Swab the rubber stopper on the peptide vial and the bacteriostatic water vial with alcohol pads. Allow them to air-dry for at least 10 seconds before proceeding.
3. Draw the water Insert the syringe needle into the bacteriostatic water vial and pull the desired volume. Remove any air bubbles by tapping the syringe and pushing them out slowly.
4. Inject slowly along the vial wall Insert the needle into the peptide vial at a slight angle. Release the water down the inside wall of the glass, not directly onto the powder cake. A slow, steady stream prevents disrupting the lyophilized material.
5. Dissolve and label Tilt and gently rotate the vial to encourage dissolution. Do not shake. The powder should dissolve into a clear solution within 1 to 2 minutes. Label the vial with the date, peptide name, and concentration. Store at 2-8C and use within 28 days.

If a solution becomes cloud or sediments occur during dissolving, please avoid using it. Properly prepared peptide solution should be clear and colorless. Please check PeptideGod BPC-157 or Real Peptides BPC-157 product page for more details on storage instructions.